Miniaturized Bioaffinity Assessment Coupled to Mass Spectrometry for Guided Purification of Bioactives
نویسندگان
چکیده
A nano-flow high-resolution screening platform, featuring a parallel chip-based microfluidic bioassay and mass spectrometry coupled to nano-liquid chromatography, was applied to screen animal venoms for nicotinic acetylcholine receptor like (nAChR) affinity by using the acetylcholine binding protein, a mimic of the nAChR. The potential of this microfluidic platform is demonstrated by profiling the Conus textile venom proteome, OPEN ACCESS
منابع مشابه
Miniaturized Bioaffinity Assessment Coupled to Mass Spectrometry for Guided Purification of Bioactives from Toad and Cone Snail
A nano-flow high-resolution screening platform, featuring a parallel chip-based microfluidic bioassay and mass spectrometry coupled to nano-liquid chromatography, was applied to screen animal venoms for nicotinic acetylcholine receptor like (nAChR) affinity by using the acetylcholine binding protein, a mimic of the nAChR. The potential of this microfluidic platform is demonstrated by profiling ...
متن کاملAnalytical workflow for rapid screening and purification of bioactives from venom proteomes.
Animal venoms are important sources for finding new pharmaceutical lead molecules. We used an analytical platform for initial rapid screening and identification of bioactive compounds from these venoms followed by fast and straightforward LC-MS only guided purification to obtain bioactives for further chemical and biological studies. The analytical platform consists of a nano-LC separation coup...
متن کاملSimultaneous Analysis of Metabolite Residues from Degradation of Dithiocarbamates in Cereals Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
Background and Objectives: Dithiocarbamates are groups of fungicides used to control pests of agricultural products. Analysis of these pesticides with liquid and gas chromatography instruments is associated with various problems due to the presence of metal groups in the structure of these compounds. Therefore, one of the ways for analysis the pesticides is to check the level of decomposition...
متن کاملMetabolic Profiling of Ligands for the Chemokine Receptor CXCR3 by Liquid Chromatography–Mass Spectrometry coupled to Bioaffinity
Metabolic profiling of ligands for the chemokine receptor CXCR3 by liquid chromatography–mass spectrometry coupled to bioaffinity assessment. Abstract Chemokine receptors belong to the class of G protein−coupled receptors and are important in the host defense against infections and inflammation. However, aberrant chemokine signaling is linked to different disorders such as cancer, central nervo...
متن کاملMetabolic profiling of ligands for the chemokine receptor CXCR3 by liquid chromatography-mass spectrometry coupled to bioaffinity assessment
Chemokine receptors belong to the class of G protein-coupled receptors and are important in the host defense against infections and inflammation. However, aberrant chemokine signaling is linked to different disorders such as cancer, central nervous system and immune disorders, and viral infections [Scholten DJ et al. (2012) Br J Pharmacol 165(6):1617-1643]. Modulating the chemokine receptor fun...
متن کامل